PQ0221

Sigma

 

ProteoQwest Chemiluminescent Western Blotting Kit, CPS Substrate

(for Rabbit Antibodies)

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Description

Features and BenefitsBenefits include
• Short procedure time
• Reproducible results
• Minimal background
General descriptionProteoQwest Chemiluminescent Western Blotting Kit, CPS Substrate, is designed for high sensitivity, low background chemiluminescent detection of as little as 0.06 ng of protein. All of the components of this ProteoQwest kit have been extensively tested and optimized. This kit is designed for 12 mini-gel sized (10 × 10 cm) blots. It is possible to use this kit for as many as 24 blots if half the suggested amount of reagents is used.
Other NotesStore the reagents as supplied in the kit at 2-8°C, except for the Chemichrome Ultimate, which should be stored in the freezer at -20°C. The unreconstituted packets and reagents are stable for 1 year. Once reconstituted, the Tris-Buffered Saline with TWEEN 20 is stable for 2 weeks at 2-8°C if filtered into a sterile container using a 0.2 μm sterile filter. Once reconstituted, the Tris-Buffered Saline with 3% Milk is stable for 1 day at 2-8°C.
Legal InformationProteoQwest is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

storage temp.2-8°C

Safety

Hazard CodesT
Risk Statements61-22-37/38-41
Safety Statements53-23-26-36/37/39-45

Components

Kit component onlyTris Buffered Saline with TWEEN® 20 12 packets
 Tris Buffered Saline, pH 8.0, with 3% nonfat Milk, powder 12 packets
 Anti-Rabbit IgG (whole molecule)-Peroxidase, ProteoQwest antibody produced in goat 150 μL
 Chemiluminescent Peroxidase Substrate-1, sufficient for 60 mL substrate 60 mL
 Chemichrome Ultimate